We have performed a comprehensive study and idntified 11,234 novel small regulatory RNAs (rsRNAs) which regulates about 17,000 unique genes. The role of these 11,234 novel rsRNAs were studied with respect to 26 different cancerous conditions data downloaded from The Cancer Genome Atlus (TCGA) and Gene Expression Omnibus (GEO) . The rsRNA:target gene interaction was identified using TAREF and Targetscan and was validated by using argonaute CLIP-sequencing data, CLASH-sequencing data, anti-coexpression using protein and RNA expresion data. form the analyses we have identified several important genes involved in regulation of cancer and many other important pathways related to cancer, growth and development, cell cycle, apoptosis and many more. The analysis performed in this study shows that these rsRNAs were originates from multiple loci and have origin in repetitive elements and intronic regions as shown from the image.